Coding
Part:BBa_K4367005:Design
Designed by: August Qvist Group: iGEM22_Chalmers-Gothenburg (2022-08-01)
MF(alpha)1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 23
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 23
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 23
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 23
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was extracted from genomic DNA. The only design consideration was when designing primers that would add overhangs to the sequence so that it could be made into a type 3 MoClo part, thereby making it MoClo compatible. No consideration was taken to making the part BioBrick compatible.
Source
This part was extracted from genomic DNA of Saccharomyces cerevisiae strain ??????.