Coding

Part:BBa_K4367005:Design

Designed by: August Qvist   Group: iGEM22_Chalmers-Gothenburg   (2022-08-01)


MF(alpha)1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 23
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was extracted from genomic DNA. The only design consideration was when designing primers that would add overhangs to the sequence so that it could be made into a type 3 MoClo part, thereby making it MoClo compatible. No consideration was taken to making the part BioBrick compatible.

Source

This part was extracted from genomic DNA of Saccharomyces cerevisiae strain ??????.

References